Anti-CD11b M1/70 抗体 （ab8878）

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Anti-CD11b [M1/70] 抗体 (ab8878)产品信息：

• Product name：Anti-CD11b [M1/70] antibody
• Description

  Rat monoclonal [M1/70] to CD11b

• Tested applicationsIHC-Fr, ICC/IF, Flow Cyt, WB
• Species reactivity

  Reacts with: Mouse, Rabbit, Human

• Immunogen

  The hybridoma was formed by the fusion of mouse myeloma NS1 cellswith spleen cells from rats immunized with B10 mouse spleen cells enrichedfor T lymphocytes.

• General notesCD11b is also known as MAC-1alpha, CR3 and integrin alpha M.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS

• Concentration 100 µg at 1 mg/ml

• PurityProtein G purified
• Clonality Monoclonal
• Clone numberM1/70
• MyelomaNS1
• IsotypeIgG2b
• Light chain typeunknown
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cell Adhesion
  • Integrins
  • Alpha
  • Tags & Cell Markers
  • Cell Type Markers
  • Neuroscience Markers
  • Glial
  • Neuroscience
  • Cell Type Marker
  • Glia marker
  • Microglia marker
  • Stem Cells
  • Mesenchymal Stem Cells
  • Negative Markers
  • Stem Cells
  • Hematopoietic Progenitors
  • Hematopoietic Stem Cells
  • Murine Lineage Negative
  • Stem Cells
  • Hematopoietic Progenitors
  • Lymphoid
  • B Lymphocytic Lineage
  • Stem Cells
  • Hematopoietic Progenitors
  • Lymphoid
  • T Lymphocytic Lineage
  • Stem Cells
  • Hematopoietic Progenitors
  • Lymphoid
  • NK Cell Lineage
  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Monocytic Lineage
  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Neutrophil Lineage

Applications

Our Abpromise guarantee covers the use of ab8878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

• FunctionIntegrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
• Tissue specificityPredominantly expressed in monocytes and granulocytes.
• Involvement in diseaseGenetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
• Sequence similaritiesBelongs to the integrin alpha chain family.
  Contains 7 FG-GAP repeats.
  Contains 1 VWFA domain.
• DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
• Cellular localizationMembrane.

• Database links

  • Entrez Gene: 3684 Human
  • Entrez Gene: 16409 Mouse
  • Omim: 120980 Human
  • SwissProt: P11215 Human
  • SwissProt: P05555 Mouse
  • Unigene: 172631 Human
  • Unigene: 262106 Mouse

• Alternative names

  • Antigen CD11b p170 antibody
  • CD11 antigen like family member B antibody
  • CD11 antigen-like family member B antibody
  • CD11b antibody
  • CD11b antigen antibody
  • CD11b/CD18 antibody
  • CD49d antibody
  • Cell surface glycoprotein MAC 1 alpha subunit antibody
  • Cell surface glycoprotein MAC-1 subunit alpha antibody
  • Cell Surface Glycoprotein MAC1 Alpha Subunit antibody
  • Complement component 3 receptor 3 subunit antibody
  • Complement Component Receptor 3 Alpha antibody
  • Complement receptor type 3, alpha subunit antibody
  • CR 3 alpha chain antibody
  • CR-3 alpha chain antibody
  • CR3 Alpha Chain antibody
  • CR3 antibody
  • CR3A antibody
  • F730045J24Rik antibody
  • Integrin Alpha M antibody
  • Integrin alpha M chain antibody
  • integrin alpha M complement component 3 receptor 3 subunit antibody
  • Integrin alpha-M antibody
  • Integrin beta 2 alpha subunit antibody
  • ITAM_HUMAN antibody
  • ITGAM antibody
  • Leukocyte adhesion receptor MO1 antibody
  • Ly-40 antibody
  • MAC 1 antibody
  • Mac-1a antibody
  • MAC1 antibody
  • Mac1, alpha subunit antibody
  • MAC1A antibody
  • Macrophage antigen alpha polypeptide antibody
  • MGC117044 antibody
  • Mo1, alpha subunit antibody
  • MO1A antibody
  • Neutrophil adherence receptor alpha M subunit antibody
  • Neutrophil adherence receptor antibody
  • SLEB6 antibody

Anti-CD11b [M1/70] antibody images

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (ab8878)

  ICC/IF image of ab8878 stained Raw 246.7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab8878 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• 

  Flow Cytometry - Anti-CD11b [M1/70] antibody (ab8878)

  Overlay histogram showing Human macrophages stained with ab8878 (blue line). Cells were incubated with human immunoglobulin for 30 min at 4°C to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8878, 20 µg/mL) for 30 min at 4ºC. The secondary antibody used was FITC mouse anti-rat IgG2b (gamma chain) (ab99671) at 1 µg/mL for 30 min at 4ºC. Isotype control antibody (black line) was Rat IgG2b [RTK4530] (ab18541) used under the same conditions.

• Flow Cytometry - Anti-CD11b [M1/70] antibody (ab8878)

  Overlay histogram showing Human macrophages stained with ab8878 (red line). Cells were pre-incubated in Human AB serum (10%) for 20 mins at 4ºC. Cells were then incubated with the antibody (ab8878, 0.1µg/1x10^6 cells) for 30 min at 4ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rat IgG (H+L) (ab150165) at 1/2000 dilution for 30 min at 4ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

• 

  Immunohistochemistry (Frozen sections) - CD11b antibody (ab8878)This image is a courtesy of Anonymous Abreview

  ab8878 staining CD11b in mouse brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocking with 5% BSA for 1 hour at 250C was performed. The sample was incubated with primary antibody (1/100) for 12 hours at 40C in PBS. A Cy3®-conjugated donkey polyclonal to rat IgG was used at dilution at 1/500 as secondary antibody. Red staining in the image represents Cy3-CD11b, green - FITC-BrdU and blue is DAPI staining.
• 

  Immunohistochemistry (PFA perfusion fixed frozen sections) - CD11b antibody [M1/70] (ab8878)This image is courtesy of an Anonymous Abreview.

  ab8878 staining CD11b in Mouse brain tissue by Immunohistochemistry (PFA perfusion fixed frozen sections). The sections were perfusion fixed with paraformaldehyde prior to blocking with TNB blocking buffer for 1 hour at 24°C. The primary antibody was diluted 1/100 in TNB blocking buffer and incubated with the sample for 24 hours at 4°C. An HRP Polymer-conjugated Goat anti-Rat polyclonal was used undiluted as the secondary antibody. Detected using a Streptavidin-Alexa 546 conjugate.

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Abcam  ab12185Rabbit polyclonal to JAB1100ul3996JAB1hystemcell一抗

Abcam  ab12187Rabbit polyclonal to MBD4100ul3996MBD4hystemcell一抗

Abcam  ab12188Rabbit polyclonal to KAT2B/PCAF - ChIP Grade50ul4418PCAFhystemcell一抗

Abcam  ab12189Mouse monoclonal [PRMT1-171] to PRMT150ul3996PRMT1hystemcell一抗

Abcam  ab12190Mouse monoclonal [PRMT3-367] to PRMT350ul3996PRMT3hystemcell一抗

Abcam  ab12191Mouse monoclonal [PRMT5-21] to PRMT550ul3996PRMT5hystemcell一抗

Abcam  ab12192Rabbit polyclonal to PRMT6100ul3996PRMT6hystemcell一抗

Abcam  ab12193Rabbit polyclonal to SIRT1100ul4274SIRT1hystemcell一抗

Abcam  ab12195Rabbit polyclonal to STAT5a50ug3996STAT5ahystemcell一抗

Abcam  ab12199Rabbit polyclonal to Ube1L100ul3996Ube1Lhystemcell一抗

Abcam  ab122Mouse monoclonal [1B4-UB] to Ubiquitin50ug3996Ubiquitinhystemcell一抗

Abcam  ab1220Mouse monoclonal [mAbcam 1220] to Histone H3 （di methyl K9） - ChIP Grade200ul4218Histone H3hystemcell一抗

Abcam  ab12200Rabbit polyclonal to Ube1L100ul3996Ube1Lhystemcell一抗

Abcam  ab12204Histone H3 peptide - di methyl K23100ug2010Histone H3蛋白/多肽多肽

Abcam  ab12206Rabbit polyclonal to Apc1 （phospho S377）100ug3996Apc1hystemcell一抗

Abcam  ab12208Rat monoclonal [94B] to HHV8 Cyclin100ug3996D type cyclin （encoded by KSV）hystemcell一抗

Abcam  ab12680Rabbit polyclonal to D650ug3996GPCR D6hystemcell一抗

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